empty control vector pcdna-nc Search Results


empty control vector pcdna-nc  (Shanghai GenePharma)
90
Shanghai GenePharma empty control vector pcdna-nc
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Empty Control Vector Pcdna Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/pcdna+empty+vector/pmc08355640-42-6-13
Average 90 stars, based on 1 article reviews
empty control vector pcdna-nc - by Bioz Stars, 2026-09
90/100 stars
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pcdna‑nc  (Shanghai GenePharma)
90
Shanghai GenePharma pcdna‑nc
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Pcdna‑Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/pcdna3+1/pm32945354-39-12-18
Average 90 stars, based on 1 article reviews
pcdna‑nc - by Bioz Stars, 2026-09
90/100 stars
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mir‑nc  (Shanghai GenePharma)
90
Shanghai GenePharma mir‑nc
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Mir‑Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/mir+nc/pm33936286-67-33-42
Average 90 stars, based on 1 article reviews
mir‑nc - by Bioz Stars, 2026-09
90/100 stars
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sirna control  (Ribobio co)
90
Ribobio co sirna control
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Sirna Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/pcdna3+1/pmc07250310-58-5-27
Average 90 stars, based on 1 article reviews
sirna control - by Bioz Stars, 2026-09
90/100 stars
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sirna targeting txndc5 si-txndc5  (Ribobio co)
90
Ribobio co sirna targeting txndc5 si-txndc5
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Sirna Targeting Txndc5 Si Txndc5, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/sirna+targeting+txndc5+si+txndc5/pm34606982-65-34-49
Average 90 stars, based on 1 article reviews
sirna targeting txndc5 si-txndc5 - by Bioz Stars, 2026-09
90/100 stars
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negative control vector (pcdna nc  (Shanghai GenePharma)
90
Shanghai GenePharma negative control vector (pcdna nc
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Negative Control Vector (Pcdna Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/si+negative+control/pm33417267-38-41-48
Average 90 stars, based on 1 article reviews
negative control vector (pcdna nc - by Bioz Stars, 2026-09
90/100 stars
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mir-20b mimics mir-20b  (Shanghai GenePharma)
90
Shanghai GenePharma mir-20b mimics mir-20b
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Mir 20b Mimics Mir 20b, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/mir+20a+5p+mimic/pm31823095-36-10-30
Average 90 stars, based on 1 article reviews
mir-20b mimics mir-20b - by Bioz Stars, 2026-09
90/100 stars
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circ_0029589 overexpression plasmid circ_0029589  (Ribobio co)
90
Ribobio co circ_0029589 overexpression plasmid circ_0029589
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Circ 0029589 Overexpression Plasmid Circ 0029589, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/circ+0091579+overexpression+plasmid++circ+0091579/pm32860985-50-25-56
Average 90 stars, based on 1 article reviews
circ_0029589 overexpression plasmid circ_0029589 - by Bioz Stars, 2026-09
90/100 stars
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negative control pcdna nc  (OriGene)
91
OriGene negative control pcdna nc
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Negative Control Pcdna Nc, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/Negative+shRNA+control+in+pGFP-V-RS+Vector/pm32888745-55-19-25
Average 91 stars, based on 1 article reviews
negative control pcdna nc - by Bioz Stars, 2026-09
91/100 stars
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pcdna-nc  (VectorBuilder GmbH)
90
VectorBuilder GmbH pcdna-nc
miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
Pcdna Nc, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+control+vector+pcdna-nc/pcdna+nc/pm35779150-39-2-10
Average 90 stars, based on 1 article reviews
pcdna-nc - by Bioz Stars, 2026-09
90/100 stars
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pcdna-ncpgcamp6s (plasmid #113674)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

Journal: Experimental and Therapeutic Medicine

Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

doi: 10.3892/etm.2021.10512

Figure Lengend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

Techniques: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control